He was extradited to the USA, where he appeared in court Tuesday before a federal judge in Brooklyn.
When the dogs arrived in the United States from Colombia, the heroin was surgically removed from their bodies, prosecutors said.
Puppies found after DEA agents arrested 21 people in Colombia charged with smuggling heroin into the United States, the agency announced February 1, 2006.
Lopez Elorez, 38, who also goes by the surname Lopez Elorza, fled in 2005 when authorities arrested about two dozen suspected traffickers in Colombia.
Assistant US Attorney Richard Donoghue said dogs were "man's best friend" and warned "we are drug dealers' worst enemy".
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"Elorza is not only a drug trafficker, he also betrayed a veterinarian's pledge to prevent animal suffering,"Richard Donoghue, for the prosecution, said". Upon delivery, the drug would be cut out of their stomachs-killing them in the process. However, two were adopted, including one that became a drug-sniffing dog for Colombian police. Authorities believe he may have been in the country for as long as eight years.
In May 2015, he went into hiding in the northwestern town of Santa Comba, after the National Court in the United States of America authorized his extradition so he could face drugs charges.
After his arrival, DEA Special Agent-in-charge James Hunt said that "over time, drug organisations" unqunechable thirst for progit leads them to do unthinkable crimes like using innocent puppies for drug concealment'.
A Colombian veterinarian is accused of implanting heroin in puppies to smuggle drugs into the United States.
If convicted on conspiracy charges, he risks spending at least 10 years and potentially life behind bars.
Elorez faces up to life imprisonment if convicted, U.S. prosecutors said.